Our new Crystal clear+ tissue clearing approach is the sole strategy that delipidates samples without improve in morphology and with nominal impact on structural integrity.
Megatome can be a vibrating microtome meant to part a wide number of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables higher-throughput tissue sectioning with uniform surface area profile, in addition to negligible tissue destruction and information loss.
Antibodies might consider weeks to diffuse by means of just a few millimeters of tissue, using a steep labeling gradient from surface area to Main.
Megatome is created for precision: the blade vibrates at an increased frequency and bigger amplitude selection than other microtomes, and incorporates a exclusive deflection Management mechanism.
SE works by using a rotational electric discipline to disperse extremely electromobile molecules (like antibodies or surfactant micelles) throughout a porous sample without harming electrically billed constructions inside the tissue. This enables 2-four working day clearing of intact organs,
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eFLASH is a swift tissue labeling method which allows for uniform complete-organ staining in twenty rounds of labeling.
The SmartSPIM mild sheet microscope gives unparalleled resolution and acquisition pace throughout intact tissue volumes, although its modular structure lets you improve and customise person parts.
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SHIELD avoids the variability of hydrogel embedding and the data reduction from PFA preservation, guarding specimens for multiple rounds of processing.
Leverage the Distinct+ tissue clearing process, coupled with eFLASH and patented stochastic electrotransport systems, to swiftly crystal clear and label whole organs. Crucial highlights and characteristics consist of:
Our novel Protect tissue preservation system types megatomi.com intramolecular bonds working with polyfunctional, versatile epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.